Sabouraud Dextrose Agar (SDA) – Uses, Composition, Preparation, Principle
Use:
Recommended for the cultivation of yeasts, moulds and aciduric bacteria from pharmaceutical products in accordance with
the microbial limit test.
Composition:
|
Ingredients |
Gms / Litre |
|
Dextrose
(Glucose) |
40.00 |
|
Mixture of
Peptone and Tryptone (1:1) |
10.00 |
|
Agar |
15.00 |
|
pH after
sterilization ( at 25°C) 5.6 ± 0.2 |
|
Preparation:
Suspend 65.0 grams in 1000 ml purified/ distilled water. Heat to boiling to dissolve the medium completely. Sterilize
by autoclaving at 15 lbs pressure (121°C) for 15 minutes or as per validated cycle. Cool to 45-50°C. Mix well and pour into
sterile Petri plates.
Principle:
Peptone and Tryptone provides carbonaceous, nitrogenous compounds, long chain amino acids, vitamins and other essential
growth nutrients. Dextrose (Glucose) provides an energy source. High dextrose concentration and low pH favors fungal
growth and inhibits contaminating bacteria from clinical specimens.
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