Mix 200 mL of 1M hydrochloric acid with 200 mL of 1M sodium acetate and dilute to 1000 mL with water. Immediately before use adjust the pH to 2.45 by the addition of 1M hydrochloric acid or 1M sodium acetate, as required.
Acetate Buffer pH 2.8
Dissolve 4 g of anhydrous sodium acetate in about 840 mL of water, add sufficient glacial acetic acid to adjust the pH to 2.8 (about 155 mL) and dilute to 1000 mL with water.
Acetate Buffer pH 3.4
Mix 5 volumes of 0.1M sodium acetate with 95 volumes of 0.1M acetic acid .
Acetate Buffer pH 3.5
Dissolve 25 g of ammonium acetate in 25 mL of water and add 38 mL of 7M hydrochloric acid. Adjust the pH to 3.5 with either 2M hydrochloric acid or 6M ammonia and dilute to 100 mL with water.
Acetate Buffer pH 3.7
Dissolve 10 g of anhydrous sodium acetate in 300 mL of water, adjust to pH 3.7 with glacial acetic acid and dilute to 1000 mL with water. If necessary, readjust to pH 3.7 with glacial acetic acid or anhydrous sodium acetate as required, before use.
Acetate Buffer pH 4.4
Dissolve 136 g of sodium acetate and 77 g of ammonium acetate in water and dilute to 1000.0 mL with the same solvent; add 250.0 mL of glacial acetic acid and mix.
Acetate Buffer pH 4.6
Dissolve 5.4 g of sodium acetate in 50 mL of water, add 2.4 g of glacial acetic acid and dilute to 100.0 mL with water. Adjust the pH if necessary.
Acetate Buffer pH 5.0
Dissolve 13.6 g of sodium acetate and 6 mL of glacial acetic acid in sufficient water to produce 1000 mL.
Acetate Buffer pH 6.0
Dissolve 100 g of ammonium acetate in 300 mL of water, add 4.1 mL of glacial acetic acid, adjust the pH if necessary using ammonia or acetic acid and dilute to 500.0 mL with water.
Acetate Buffer Solution pH 4.5
Dissolve 77.1 g of ammonium acetate in water. Add 70 mL of glacial acetic acid and dilute to 1000.0 mL with water.
Acetate Buffer Solution pH 4.7
Dissolve 136.1 g of sodium acetate in 500 mL of water. Mix 250 mL of this solution with 250 mL of dilute acetic acid. Shake twice with a freshly prepared, filtered, 0.1 g/L solution of dithizone in chloroform. Shake with carbon tetrachloride until the extract is colourless. Filter the aqueous layer to remove traces of carbon tetrachloride.
Acetate Buffer Solution pH 4.7 R1
Dissolve 136.1 g of sodium acetate in 500 mL of water. Mix 250 mL of this solution with 250 mL of dilute acetic acid.
Acetate Buffer Solution pH 5.0
To 120 mL of a 6 g/L solution of glacial acetic acid add 100 mL of 0.1M potassium hydroxide and about 250 mL of water. Mix. Adjust the pH to 5.0 with a 6 g/L solution of acetic acid or with 0.1M potassium hydroxide and dilute to 1000.0 mL with water.
Acetate–edetate Buffer Solution pH 5.5
Dissolve 250 g of ammonium acetate and 15 g sodium edetate in 400 mL of water and add 125 mL of glacial acetic acid.
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