Soyabean Casein Digest Agar (Tryptone Soya Agar / Casein Soyabean Digest Agar) use, principle and composition.
Soyabean Casein Digest Agar is a general purpose medium used for cultivation of a wide variety of microorganisms and for sterility testing in pharmaceutical procedures.
Principle:
Soyabean Casein Digest Agar is a widely used medium, which supports the growth of wide variety of organisms even that of fastidious ones such as Neisseria, Listeria, and Brucella etc.
The medium with addition of blood provides perfectly defined haemolysis zones, while preventing the lysis of erythrocytes due to its sodium chloride content.
It has been frequently used in the health industry to produce antigens, toxins etc. Its simple and inhibitor-free composition makes it suitable for the detection of antimicrobial agents in the food and other products.
Tryptone Soya Agar is recommended by various pharmacopoeias as sterility testing medium.Tryptone Soya Agar conforms as per USP and is used in microbial limit test and antimicrobial preservative - effective test.Gunn used this medium for the growth of fastidious organisms and study of haemolytic reaction after addition of 5% v/v blood.
The combination of tryptone and soya peptone makes this media nutritious by providing amino acids and long chain peptides for the growth of microorganisms. Sodium chloride maintains the osmotic balance.
Composition:
Ingredients
|
Gms / Litre
|
Tryptone
|
15.0
|
Soya peptone
|
5.0
|
Sodium chloride
|
5.0
|
Agar
|
15.0
|
Final pH ( at 25°C) 7.3±0.2
|
|
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